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Below is the standard protocol for peptide desalting and concentration using C18 ZipTips. 🧪 C18 ZipTip Standard Protocol 1. Preparation & Materials

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Repeat . This prepares the resin to bind the peptides. 4. Sample Binding Below is the standard protocol for peptide desalting

💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry. Repeat

Dispense the liquid into a clean, sterile microcentrifuge tube.

50–70% ACN in 0.1% TFA (depending on peptide hydrophobicity). 2. Wetting the Tip Set your pipette to 10 µL. Aspirate the Wetting Solution (100% ACN) into the tip. Dispense to waste. Repeat 2 times . This activates the C18 resin. 3. Equilibration Aspirate the Equilibration Solution (0.1% TFA). Dispense to waste.

The sample is now ready for (MALDI-TOF or LC-MS/MS).