: The episode's structure and the use of a "test" to find a hidden monster among friends heavily mirror the 1982 horror film.
: The title is a direct nod to the 1939 mystery novel where guests on an island are killed off one by one.
In this episode, Sam, Dean, and Bobby investigate a series of brutal murders that lead them to a cannery where they encounter fellow hunters, including , Samuel Campbell , and Gwen Campbell . They discover that Eve (the Mother of All) has created a new supernatural parasite known as the Khan Worm . Key Plot "Features"
The reference refers to the sixteenth episode of the sixth season of the TV series Supernatural , which first aired on March 4, 2011. Episode Overview
: This episode is notable for killing off recurring characters Rufus Turner , Samuel Campbell , and Gwen Campbell .